General Cell Based Assays
Cell Biolabs Inc CytoSelect™ 96-Well Cell Migration Assay Kit (3 µm, Fluorometric), 5x96 assays
Chemotaxis, a type of cell migration, is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia that are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions. The CytoSelect™ Cell Migration Assay Kit utilizes polycarbonate membrane inserts (8 µm pore size) to assay the migratory properties of cells. The kit contains 5x96 polycarbonate membrane inserts with 3 um pores in each of five 96-well plates, through which migratory cells are able to pass. Cells are quantified using the provided fluorometric dye. The 3 µm pore size is optimal for measuring the migration of leukocytes.
Eurofins Discoverx PathHunter eXpress MC3R Total Internalization Assay, 10pl Kit. (93-0837E3CP0L)
The PathHunter MC3R Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of MC3R via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged MC3R and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the MC3R receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL4R/IL2RG Dimerization Assay, 10pl Kit. (93-0988E3CP5L)
The PathHunter IL4R/IL2RG Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-4) induced dimerization of IL4R/IL2RG receptors. This product uses EFC technology. The cell line included overexpresses IL4R target protein fused to PK alongside IL2RG target protein fused to EA. Upon ligand-induced dimerization of IL4R/IL2RG, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Microsolv Technology Corporation Base Plate, Translucent Polypropylene, Micro-Sample Management System, universal, re-usable. 12/CS.
Accommodates and holds one Micro-Sample Insert Rack in the U-2D System. Made of low weight polypropylene plastic, they comply with ANSI standards for 96 well plates. When used with Racks of Inserts, the unique molded bottoms allow each insert to be supported on the bottom. Use in a vortex mixer or water bath can be used with enhanced performance. Also most plate readers can be used. Bases are reusable and can be refrigerated.
Eurofins Discoverx PathHunter eXpress ErbB2/ErbB4 Dimerization Assay, 2pl Kit. (93-0960E3CP5M)
The PathHunter ErbB2/ErbB4 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. heregulin β-1) induced dimerization of ErbB2/ErbB4 receptors. This product uses EFC technology. The cell line included overexpresses ErbB2 target protein fused to PK alongside ErbB4 target protein fused to EA. Upon ligand-induced dimerization of ErbB2/ErbB4, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTHR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0306E2CP0M)
The PathHunter PTHR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PTH) based activation of PTHR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTHR2 and EA-tagged β-Arrestin-2. Activation of PTHR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Innovative Research Inc Rat Act tPA Kit
Rat Active tPA ELISA Kit - This rat tPA activity assay is intended for the quantitative determination of active tissue plasminogen activator (tPA) in rat plasma and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. Functionally active rat tPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Only free active tPA will react with the PAI-1 bound to the plate. After appropriate washing steps, polyclonal anti-tPA primary antibody binds to the captured tPA. Excess antibody is washed away and bound primary antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of active tPA in the sample.
Eurofins Discoverx PathHunter eXpress GPR149 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0420E2ACP1M)
The PathHunter GPR149 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR149 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR149 and EA-tagged β-Arrestin-2. Activation of GPR149 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cellular Dynamics International iCell Motor Neurons Kit, R1051, 01279, human iPSC-derived spinal motor neurons, ≥1.0 x 10^6 viable cells per vial, cryopreserved, includes base medium 100 ml, supplement A 2 ml, nervous system supplement 1 ml
iCell Motor Neurons Kit, R1051, 01279, human iPSC-derived spinal motor neurons, ≥1.0 x 10^6 viable cells per vial, cryopreserved, includes base medium 100 ml, supplement A 2 ml, nervous system supplement 1 ml
Eurofins Discoverx cAMP Hunter eXpress FPR3 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0171E2CP2L)
The cAMP Hunter FPR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. humanin) based activation of FPR3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type FPR3, a naturally Gi/Go coupled GPCR. Upon activation of FPR3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX AB38 4G8 ASY
The Meso Scale Discovery R-PLEX Human Abeta38 (4G8) Assay Kit is a multiplex immunoassay that measures Abeta38 in human serum plasma CSF and cell culture supernatants It uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay kit includes a plate coated with a 4G8 antibody to capture Abeta38 It also includes detection antibodies standards buffers calibrators controls and substrate The kit is for research use only and not for use in diagnostic procedures It allows sensitive accurate and reproducible quantification of Abeta38 levels
Eurofins Discoverx PathHunter eXpress GPR30 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0373E2ACP2M)
The PathHunter GPR30 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR30 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR30 and EA-tagged β-Arrestin-2. Activation of GPR30 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 15 Reagent, 100. (93-0563R15A)
AssayComplete Cell Plating 15 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx cAMP Hunter eXpress GPBAR1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0049E2CP2M)
The cAMP Hunter GPBAR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPBAR1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GPBAR1, a naturally Gs coupled GPCR. Upon activation of GPBAR1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.